ENO2/NSE antibody

Catalog No. ABIN7466748

This Rabbit Polyclonal antibody specifically detects ENO2/NSE in WB, IF, IHC (p), ICC and IHC (fro). It exhibits reactivity toward Human.

Quick Overview for ENO2/NSE antibody (ABIN7466748)

Target: ENO2/NSE (ENO2) (Enolase 2 (Gamma, Neuronal) (ENO2))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ENO2/NSE antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-ENO2/NSE Antibody

Specificity: This antibody was raised against human NSE (ENO2) protein, but it may cross react other family members based on sequence homology.

Cross-Reactivity: Human, Mouse, Rat, Zebrafish (Danio rerio)

Purification: Purified by antigen-affinity chromatography.

Immunogen: Recombinant protein encompassing a sequence within the center region of human NSE. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: WB: 1:1000-1:10000. ICC/IF: 1:100-1:1000. IHC-Fr: 1:100-1:1000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Comment:

Positive Control: Mouse brain , 293T , A431

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: 0.1M Tris-Glycine ( pH 7), 10 % Glycerol, 0.01 % Thimerosal

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Target Details for ENO2/NSE

Target: ENO2/NSE (ENO2) (Enolase 2 (Gamma, Neuronal) (ENO2))

Alternative Name: enolase 2

Background: Enolase 2 , HEL-S-279 , NSE,This gene encodes one of the three enolase isoenzymes found in mammals. This isoenzyme, a homodimer, is found in mature neurons and cells of neuronal origin. A switch from alpha enolase to gamma enolase occurs in neural tissue during development in rats and primates. [provided by RefSeq]

Molecular Weight: 47 kDa

Gene ID: 2026

UniProt: P09104